Prospects of silver nanoparticles (AgNPs) synthesized by Justicia secunda aqueous extracts on diabetes and its related complications

Olasunkanmi K. Awote, Mutiu I. Kazeem, Anthony B. Ojekale, Oyinkansolami B. Ayanleye, Hamdalat T. Ramoni


The wide range of secondary metabolites in medicinal plant extracts are used to treat and manage various diseases worldwide but more recently plant-mediated bio-nanotechnology is rapidly generating great research interest. Herein, the in-vitro study of the antidiabetic, anti-inflammatory, antiglycation, and antioxidant potentials of silver nanoparticles (AgNPs) synthesized by Justicia secunda aqueous extracts was evaluated. The synthesized AgNPs was characterized by UV-visible spectrophotometer, Fourier transform infrared spectroscopy (FT-IR) and Scanning electron microscopy (SEM). The antioxidant activity was evaluated via reducing power, 2,2-diphenyl-1-picrylhydrazyl (DPPH), total antioxidant capacity (TAC) and nitric oxide scavenging assays; antidiabetic activity was evaluated with α-glucosidase and α-amylase inhibitory assays; antiglycation activity was evaluated using fructosamine inhibitory assay, and anti-inflammatory activity was performed using trypsin inhibitory and albumin denaturation assays. The AgNPs from all the plant parts showed good biological potentials investigated compared to the plant extracts however, the synthesized AgNPs using the leaf aqueous extract (AgNPs-JsL) showed a better antioxidant, antidiabetic, antiglycation, and anti-inflammatory potentials with an essential quality absorption band in the ultraviolet region of 410 nm and average size distribution of 50.47nm. In conclusion, all the parts of J. secunda showed substantial antidiabetic, antioxidant, antiglycation and anti-inflammatory potentials with high yield of various bioactive metabolites, however, the AgNPs-JsL was more potent followed by the flower, stem and roots extracts, respectively.

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AlAmri OD, Albeltagy RS, Akabawy AM, Mahgoub S, Abdel-Mohsen DM, Moneim AEA, & Amin HK (2020). Investigation of antioxidant and anti-inflammatory activities as well as the renal protective potential of green coffee extract in high fat-diet/streptozotocin-induced diabetes in male albino rats. Journal of functional foods. 71:103996.

Ifedibalu Chukwu EI, Aparoop D, & Kamaruz Z (2020). Antidiabetic, anthelmintic and antioxidation properties of novel and new phytocompounds isolated from the methanolic stem-bark of Vernonia amygdalina Delile (Asteraceae). Scientific African. 10:e00578.

Oguntibeju OO (2019). Hypoglycaemic and anti-diabetic activity of selected African medicinal plants. International journal of physiology, pathophysiology and pharmacology. 11(6):224.

Singh VP, Bali A, Singh N, & Jaggi AS (2014). Advanced glycation end products and diabetic complications. The Korean Journal Physiology and Pharmacology. 18(1):1-14.

Sifuentes-Franco S, Padilla-Tejeda DE, Carrillo-Ibarra S, & Miranda-Díaz AG (2018). Oxidative stress, apoptosis, and mitochondrial function in diabetic nephropathy. International Journal of endocrinology. Article ID 1875870.

Ray A, Huisman M, Tamsma J, Van Asten J, Bingen B, Broeders E, Hoogeveen E, Van Hout F, Kwee V, & Laman B (2009). The role of inflammation on atherosclerosis, intermediate and clinical cardiovascular endpoints in type 2 diabetes mellitus. European Journal of Internal Medicine. 20(3):253-60.

Vasarri M, Barletta E, Ramazzotti M, & Degl’Innocenti D (2020). In vitro anti-glycation activity of the marine plant Posidonia oceanica (L.) Delile. Journal of Ethnopharmacology. 259:112960.

Sharma V, Gupta M, Chauhan DN, Chauhan NS, Goyal RK, & Shah K (2019). Novel Drug Delivery Systems for Phytoconstituents: Current and Future Prospects. Novel Drug Delivery Systems for Phytoconstituents. CRC Press, 3-7.

Prabhakar PK & Doble M (2011). Mechanism of action of natural products used in the treatment of diabetes mellitus. Chinese Journal of Integrative Medicine. 17(8):563-74.

Verma S, Gupta M, Popli H, & Aggarwal G (2018). Diabetes mellitus treatment using herbal drugs. International Journal of Phytomedicine. 10(1):1-10.

Onoja SO, Ezeja MI, Omeh YN, & Onwukwe BC (2017). Antioxidant, anti-inflammatory and antinociceptive activities of methanolic extract of Justicia secunda Vahl leaf. Alexandria Journal of Medicine. 53(3):207-13.

Behravan M, Panahi AH, Naghizadeh A, Ziaee M, Mahdavi R, & Mirzapour A (2019). Facile green synthesis of silver nanoparticles using Berberis vulgaris leaf and root aqueous extract and its antibacterial activity. International Journal of Biological Macromolecules. 124:148-54.

Abdullah JAA, Eddine LS, Abderrhmane B, Alonso-González M, Guerrero A, & Romero A (2020). Green synthesis and characterization of iron oxide nanoparticles by Pheonix dactylifera leaf extract and evaluation of their antioxidant activity. Sustainable Chemistry and Pharmarcy. 17:100280.

Thakur S, Sharma S, Thakur S, & Rai R (2018). Green synthesis of copper nano-particles using Asparagus adscendens roxb. Root and leaf extract and their antimicrobial activities. International Journal of Current Microbiology and Applied Science. 7(4):683-94.

Menon S, Agarwal H, Kumar SR, & Kumar SV (2017). Green synthesis of silver nanoparticles using medicinal plant Acalypha indica leaf extracts and its application as an antioxidant and antimicrobial agent against foodborne pathogens. International Journal of Applied Pharmaceutics. 9(5):42-50.

Li X, Xu H, Chen Z-S, & Chen G (2011). Biosynthesis of nanoparticles by microorganisms and their applications. Journal of Nanomaterials. 8: 1-16.

Awote OK, Adeyemo AG, Igbalaye JO, Awosemo RB, Ibrahim AB, Omolaja BE, Fidausi A, & Fajobi T (2021). In Vitro Alpha-Amylase Inhibitory Activity, Antioxidant Activity and HPLC Analysis of Eichhornia crassipes (water hyacinth) Methanol Extracts. Tropical Journal of Natural Products Research. 5(12):2174-81.

Christensen L, Vivekanandhan S, Misra M, & Kumar Mohanty A. Biosynthesis of silver nanoparticles using Murraya koenigii (curry leaf): an investigation on the effect of broth concentration in reduction mechanism and particle size. Advanced Materials Letters. 2(6):429-34.

Ali ZA, Yahya R, Sekaran SD, & Puteh R (2016). Green synthesis of silver nanoparticles using apple extract and its antibacterial properties. Advances in Materials Science and Engineering. Article ID 4102196.

Sofowora A (1996). Research on medicinal plants and traditional medicine in Africa. Journal of Alternative and Complementary Medicines. 2(3): .365-372.

Ojewunmi O, Oshodi T, Ogundele O, Chijioke M, & Adenekan S (2013). Toxicity screening and in vitro antioxidant activities of aqueous extracts of Morinda lucida and Saccharum officinarum leaves. Biokemistri. 25 (2): 72-78.

Sulaimon L, Anise E, Obuotor E, Samuel T, Moshood A, Olajide M, & Fatoke T (2020). In vitro antidiabetic potentials, antioxidant activities and phytochemical profile of african black pepper (Piper guineense). Clinical Phytoscience. 2020;6(1):1-13.

Safari MR, Azizi O, Heidary SS, Kheiripour N, & Ravan AP (2018). Antiglycation and antioxidant activity of four Iranian medical plant extracts. Journal of Pharmacopuncture. 21(2):82.

Govindappa M, Hemashekhar B, Arthikala M-K, Rai VR, & Ramachandra Y (2018). Characterization, antibacterial, antioxidant, antidiabetic, anti-inflammatory and antityrosinase activity of green synthesized silver nanoparticles using Calophyllum tomentosum leaves extract. Results in Physics. 9:400-408.

Jain S & Mehata MS (2017). Medicinal plant leaf extract and pure flavonoid mediated green synthesis of silver nanoparticles and their enhanced antibacterial property. Scientific Reports. 7(1):1-13.

Vasireddy R, Paul R, & Mitra AK (2012). Green synthesis of silver nanoparticles and the study of optical properties. Nanomaterials and Nanotechnology.. 2:2-8.

Chokshi K, Pancha I, Ghosh T, Paliwal C, Maurya R, Ghosh A, & Mishra S (2016). Green synthesis, characterization and antioxidant potential of silver nanoparticles biosynthesized from de-oiled biomass of thermotolerant oleaginous microalgae Acutodesmus dimorphus. RSC Advances. 6(76):72269-74.

Newman DJ & Cragg GM (2020). Natural products as sources of new drugs over the nearly four decades from 01/1981 to 09/2019. Journal of Natural Products. 83(3):770-803.

Anyasor GN, Moses N, & Kale O (2020). Hepatoprotective and hematological effects of Justicia secunda Vahl leaves on carbon tetrachloride induced toxicity in rats. Biotechnic and Histochemistry. 95(5):349-59.

Souto AL, Tavares JF, Da Silva MS, Diniz MdFFM, Athayde-Filho D, Filgueiras P, & Barbosa Filho JM (2011). Anti-inflammatory activity of alkaloids: an update from 2000 to 2010. Molecules. 16(10):8515-34.

Lu JJ, Bao JL, Chen XP, Huang M, & Wang YT (2012). Alkaloids isolated from natural herbs as the anticancer agents. Evidence-based complementary and alternative medicine. Article ID 485042.

Bolignano D, Cernaro V, Gembillo G, Baggetta R, Buemi M, & D’Arrigo G (2017). Antioxidant agents for delaying diabetic kidney disease progression: A systematic review and meta-analysis. PloS one. 12(6):e0178699.

Chavan C, Mulik S, Chavan M, Adnaik R, Patil P (2011). Screening of antioxidant activity and phenolic content of whole plant of Barleria prionitis Linn. International Journal of Research in Ayurveda & Pharmarcy. 2(4): 1313-1319.

Rondeau P & Bourdon E (2011). The glycation of albumin: structural and functional impacts. Biochimie. 93(4):645-58. 35. Zhang L, Ravipati AS, Koyyalamudi SR, Jeong SC, Reddy N, Smith PT, Bartlett J, Shanmugam K, Münch G, & Wu MJ (2011). Antioxidant and anti-inflammatory activities of selected medicinal plants containing phenolic and flavonoid compounds. J. Agric Food Chem. 59(23):12361-7.


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