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Electrochemical quantification of the levels of hydrogen peroxide in cassava using glassy carbon electrode modified with chitosan/silver nano-hybrid

A. A. Akintoye(1), G. O. Ibrahim(2), W. O. Okiei(3),


(1) University of Lagos
(2) University of Lagos.
(3) University of Lagos
Corresponding Author

Abstract


This study correlates the production of hydrogen peroxide in cassava with its rapid postharvest physiological deterioration (PPD). Chitosan/silver nanohybrid was synthesized and immobilized on glassy carbon electrode for improved detection of hydrogen peroxide in electrochemical studies. The cathodic peak current for the reduction of hydrogen peroxide to water and oxygen occurred at -550 mV and β-carotene contents of the cassava cultivars were quantified using UV-Vis spectroscopy at a wavelength of 480 nm. No significant amount of hydrogen peroxide was found in the root tubers on the first, second, third and fourth day. However, the production of hydrogen peroxide from the different cultivars on the fifth and sixth day after harvest was found to correlate with their respective β-carotene contents. The cultivar with the highest β-carotene content (Yellow roots- IBA070593: 0.0044 mg/g) was found to have the lowest level of hydrogen peroxide on day 5 and day 6: 0.096 mmol/g FW and 0.037 mmol/g respectively; while that with the least β-carotene level (White roots- IBA980505: 0.0000 mg/g) demonstrated the highest level of hydrogen peroxide content on day 5 and day 6: 0.177 mmol/g FW and 0.096 mmol/g respectively; and highest percentage increase from the fifth to the sixth day of the PPD process. It is seen from this study that an increase in the level of hydrogen peroxide indicates PPD, and that antioxidants with hydrogen peroxide scavenging properties can help increase shelf-life of cassava cultivars

Keywords


Cassava, food security, postharvest physiological deterioration

References


Atanda SA, Pessu P, Ihionu GC, & Oladeji OO (2019). Preparation and characterization of chitosan-silver nanoparticle. NISEB Journal, 13: 3-4.

Best R (1990). Storage and processing of cassava in Latin America, lessons for the subregion (West Africa). Action Programme for the Prevention of Food Losses, Improving Post-Harvest Handling, Storage and Processing of Root and Tuber Crops; FAO: Rome, Italy, 1990; pp 9-20.

Blagbrough IS, Bayoumi SA, Rowan MG, & Beeching JR (2010). Cassava: an appraisal of its phytochemistry and its biotechnological prospects. Phytochemistry, 71: 17-18.

Booth RH, De Buckle TS, Cardenas OS, Gomez G, & Hervas E (1976). Changes in quality of cassava roots during storage. International Journal of Food Science & Technology, 11(3): 245-264.

Buschmann H, Reilly K, Rodriguez MX, Tohme J, & Beeching JR (2000). Hydrogen peroxide and flavan-3-ols in storage roots of cassava (manihot esculenta crantz) during postharvest deterioration. Journal of Agricultural and Food Chemistry, 48(11): 5522-5529.

Buschmann H, Tohme J, & Beeching JR (2004). Oxidative stress responses during cassava post-harvest physiological deterioration. Plant Mol Biol 56: 625–641.

Cock JH (1985). Cassava. New potential for a neglected crop. IADS/Westview Press, Boulder Colorado, pp 192.

Dai D, Hu Z, Pu G, Li H, & Wang C (2006). Energy efficiency and potentials of cassava fuel ethanol in Guangxi region of China. Energy Conversion and Management, 47(13-14): 1686-1699.

De Bruijn GH & Fresco LO (1989). The importance of cassava in world food production. Netherlands Journal of Agricultural Science, 37: 21-34.

Drapcho CM, Nhuan NP, & Walker TH (2008). Biofuels engineering process technology (No. Sirsi) i9780071487498). New York: McGraw-Hill.

Govindan S, Nivethaa EAK, Saravanan R, Narayanan V, & Stephen A (2012). Synthesis and characterization of chitosan–silver nanocomposite. Applied Nanoscience, 2(3): 299-303. Harborne J B (1973). Phenolic compounds. Phytochemical methods, pp. 33-88.

Hirose S, Data ES, Quevedo MA. Changes in respiration and ethylene production in cassava roots in relation to postharvest deterioration (1984). Tropical Root Crops: Postharvest Physiology and Processing; pp 83-98.

Huang J, Bachem C, Jacobsen E, & Visser RG (2001). Molecular analysis of differentially expressed genes during postharvest deterioration in cassava (Manihot esculenta Crantz) tuberous roots. Euphytica, 120(1): 85-93.

Iyer S, Mattinson DS, and Fellman JK (2010). Study of the early events leading to cassava root postharvest deterioration. Tropical Plant Biology, 3(3): 151-165.

Kawano K (1980). Cassava. Hybridization of crop plants, 225-233. Lalaguna F & Agudo M (1989). Relationship between changes in lipid with ageing of cassava roots and senescence parameters. Phytochemistry, 28(8): 2059-2062.

Li S, Cui Y, Zhou Y, Luo Z, Liu J, & Zhao M (2017). The industrial applications of cassava: current status, opportunities and prospects. Journal of the Science of Food and Agriculture, 97(8): 2282-2290.

Montagnac JA, Davis CR, & Tanumihardjo S A. (2009a). Nutritional value of cassava for use as a staple food and recent advances for improvement. Comprehensive Reviews in Food Science and Food Safety, 8(3): 181–194.

Montagnac JA, Davis CR, & Tanumihardjo SA (2009b). Processing techniques to reduce toxicity and antinutrients of cassava for use as a staple food. Comprehensive Reviews in Food Science and Food Safety, 8(1): 17-27.

Nassar N & Ortiz R (2010). Breeding cassava to feed the poor. Scientific American, 302(5): 78-85.

Olaniyan OJ, Dare EO, Adetunji OR, Adedeji OO, & Ogungbesan SO (2016). Synthesis and characterization of chitosan-silver nanocomposite film. Nano Hybrids and Composites, 11: 22-29.

Oluwole OB, Olatunji OO, & Odunfa SA (2004). A process technology for conversion of dried cassava chips into ―Gari‖. Nigerian Food Journal, 22(1): 65-77.

Priya R & Siva R (2014). Phylogenetic analysis and evolutionary studies of plant carotenoid cleavage dioxygenase gene. Gene, 548(2): 223-233.

Qian RQ & Glanville RW (2005). U.S. Patent No. 6,896,809. Washington, DC: U.S. Patent and Trademark Office

Reilly K, Gómez-Vásquez R, Buschmann H, Tohme J, Beeching JR (2004). Oxidative stress responses during cassava post-harvest physiological deterioration. Plant Mol Biol 56: 625–641.

Rhim JW, Hong SI, Park HM, & Ng PK (2006). Preparation and characterization of chitosan-based nanocomposite films with antimicrobial activity. Journal of agricultural and food chemistry, 54(16): 5814-5822.

Rickard J E (1985). Physiological deterioration of cassava roots. Journal of the Science of Food and Agriculture, 36(3): 167-176.

Rudi N, Norton GW, Alwang JR, & Asumugha GN. (2010). Economic impact analysis of marker-assisted breeding for resistance to pests and post-harvest deterioration in cassava. African Journal of Agricultural and Resource Economics, 4(311): 110-122.

Sánchez T, Chávez AL, Ceballos H, Rodriguez‐Amaya DB, Nestel P, & Ishitani, M. (2006). Reduction or delay of post‐harvest physiological deterioration in cassava roots with higher carotenoid content. Journal of the Science of Food and Agriculture, 86(4): 634-639.

Sandeep N (2017). Effect of aligned magnetic field on liquid thin film flow of magnetic-nanofluids embedded with graphene nanoparticles. Advanced Powder Technology, 28(3): 865-875.

Saxena A, Tripathi RM, Zafar F, & Singh P (2012). Green synthesis of silver nanoparticles using aqueous solution of Ficus benghalensis leaf extract and characterization of their antibacterial activity. Materials letters, 67(1): 91-94.

Sharma VK, Yngard RA, & Lin Y (2009). Silver nanoparticles: green synthesis and their antimicrobial activities. Advances in colloid and interface science, 145(1-2): 83-96.

Simonyan KJ. (2014). Cassava post-harvest processing and storage in Nigeria: A review. African Journal of Agricultural Research, 9(53): 3853-3863.

Tonukari NJ (2004). Cassava and the future of starch. Journal of Biotechnology, 7(1): 5-8.

Tran HV, Dai Tran L, Ba CT, Vu HD, Nguyen TN, Pham DG, & Nguyen PX (2010). Synthesis, characterization, antibacterial and antiproliferative activities of monodisperse chitosan-based silver nanoparticles. Colloids and surfaces A: Physicochemical and Engineering Aspects, 360: 1-3.

Uarrota VG, Moresco R, Schmidt EC, Bouzon ZL, Da Costa Nunes E, De Oliveira Neubert E, & Maraschin M (2016). The role of ascorbate peroxidase, guaiacol peroxidase, and polysaccharides in cassava (manihot esculenta crantz) roots under postharvest physiological deterioration. Food Chemistry, 197: 737-746.

Uritani I (1998). Biochemical comparison in storage: Stress response between sweet potato and cassava. Tropical Agriculture-London Then Trinidad-, 75: 177-182.

Zargar M, Shameli K, Najafi GR, & Farahani F (2014). Plant mediated green biosynthesis of silver nanoparticles using Vitex negundo L. extract. Journal of Industrial and Engineering Chemistry, 20(6): 4169-4175.

Zidenga T, Leyva-Guerrero E, Moon H, Siritunga D, & Sayre R (2012). Extending cassava root shelf life via reduction of reactive oxygen species production. Plant physiology, 159(4): 1396-1407.


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DOI: 10.57046/ZZBE4788

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