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Original Research Article - Isolation of pathogenic strains of methicillin-resistant Staphylococcus aureus from mobile phones in a tertiary institution in Ibadan, Nigeria

Olumuyiwa S. Alabi(1), Oluwatobi O. Ayoola(2), Jesutofunmi S. Odeyemi(3), Anderson O. Oaikhena(4),


(1) University of Ibadan
(2) University of Ibadan
(3) University of Ibadan
(4) University of Ibadan
Corresponding Author

Abstract


The health implications of mobile phones emission have been a matter of concern to health practitioners for decades but little or no attention has been given to the mobile phones as potential fomites for pathogens. This study isolated and characterised methicillin-resistant Staphylococcus aureus (MRSA) on mobile phones of students in a tertiary institution in Ibadan, Nigeria. One hundred swab samples were obtained from the surfaces of mobile phones of students in a cross-sectional study conducted between July and August, 2019. Presumptive staphylococci were isolated on mannitol salt agar and identities were confirmed by standard biochemical test and Gram-staining. Antimicrobial susceptibility test was done by disc-diffusion method and mecA or mecALGA251, tsst-1, eta and etb genes screened by PCR technique. Among the 200 presumptive S. aureus isolated, 163 (81.5%) were confirmed as S. aureus exhibiting resistance to ofloxacin (1.8%), gentamicin (3.7%), ceftriaxone (29.4%), ceftazidime (57.7%), cefoxitin (61.3%), cefuroxime (64.4%), erythromycin (85.3%), amoxicillin-clavulanic acid (86.5%) and cloxacillin (92%). Proportion of MRSA and methicillin-susceptible S. aureus (MSSA) were 61.3% and 38.7% respectively with significant difference in their level of antibiotic resistance to the cephalosporins (excluding ceftriaxone), penicillins, erythromycin and ofloxacin (p<0.05). All the MRSA strains were multidrug resistant (MDR) harbouring mecA or mecALGA251 (56.3%), tsst-1 (56.3%), etb (31.3%) and eta (12.5%) genes. This study detected a high proportion of methicillin-resistant, virulent S. aureus as fomites on surfaces of mobile phones among students of a tertiary institution. This is of public health concern and thus requires strong public education to avoid imminent spread and outbreaks.

Keywords


MRSA; mobile phone; eta; etb; tsst-1; mecA; mecALGA251

References


Adejoro DO, Ayodele OC, Oke AM, & Arogundade AA (2019). Phone usage patterns and bacterial contamination of mobile phones of Adekunle Ajasin University, Akungba-Akoko students. World Rural Observations. 11(3): 33-39.

Adhikari S, Khadka S, Sapkota S, & Shrestha P (2018). Methicillin-resistant Staphylococcus aureus associated with mobile phones. SOJ Microbiology and Infectious Diseases. 6(1): 1-6.

Akinyemi KO, Atapu AD, Adetona OO, & Coker AO (2009). The potential role of mobile phones in the spread of bacterial infections. The Journal of Infection in Developing Countries. 3(08): 628-632.

Aklilu E & Hui-Ying C (2020). First mecC and mecA positive livestock-associated methicillin resistant Staphylococcus aureus (mecC MRSA/LA-MRSA) from dairy cattle in Malaysia. Microorganisms. 8(2): 147.

Al-Ghamdi AK, Ashshi SAA, Faidah H, Shukri H, & Jiman-Fatani AA (2011). Bacterial contamination of computer keyboards and mice, elevator buttons and shopping carts. African Journal of Microbiology Research. 5(23): 3998-4003.

Alli OA, Ogbolu DO, Shittu AO, Okorie AN, Akinola JO, & Daniel JB (2015). Association of virulence genes with mecA gene in Staphylococcus aureus isolates from tertiary hospitals in Nigeria. Indian Journal of Pathology and Microbiology. 58: 464–471.

Archer NK, Mazaitis MJ, Costerton JW, Leid JG, Powers ME, & Shirtliff ME (2011). Staphylococcus aureus biofilms: properties, regulation, and roles in human disease. Virulence. 2(5): 445-459. DOI: 10.4161/viru.2.5.17724

Arifin WN (2013). Introduction to sample size calculation. Education in Medicine Journal. 5(2). Available at: https://eduimed.usm.my/EIMJ20130502/EIMJ20130502_12.pdf

Ayeni FA, Gbarabon T, Andersen C, & Nørskov-Lauritsen N (2015). Comparison of identification and antimicrobial resistance pattern of Staphylococcus aureus isolated from Amassoma, Bayelsa state, Nigeria. African Health Sciences. 15(4): 1282-1288.

Ba X, Harrison EM, Lovering AL, Gleadall N, Zadoks R, Parkhill J, Peacock SJ, Holden MT G, Paterson GK, & Holmes MA (2015). Old drugs to treat resistant bugs: methicillin-resistant Staphylococcus aureus isolates with mecC are susceptible to a combination of penicillin and clavulanic acid, Antimicrobial Agents and Chemotherapy. 59 (12): 7396-7404. Doi: 10.1128/AAC.01469-15.

Bietrix J, Kolenda C, Sapin A, Haenni M, Madec J Y, Bes M, Dupieux C, Tasse J, & Laurent F (2019). Persistence and diffusion of mecC-positive CC130 MRSA isolates in dairy farms in meurthe-et-moselle county (France). Frontiers in Microbiology. 10: 47. Doi: 10.3389/fmicb.2019.00047.

Brady RR, Fraser SF, Dunlop MG, Paterson-Brown S & Gibb AP (2007). Bacterial contamination of mobile communication devices in the operative environment. Journal of Hospital Infection. 66(4): 397-398.

Cheesbrough M (2017). District Laboratory Practice in Tropical Countries. Part 2, Second edition. Cambridge: Cambridge University Press pp 62 -70.

Choo EJ (2017). Community-associated methicillin-resistant Staphylococcus aureus in nosocomial infections. Infection and Chemotherapy. 49(2): 158–159.

Ciesielczuk H, Xenophontos M, & Lambourne J (2019). Methicillin-resistant Staphylococcus aureus harboring mecC still eludes us in East London, United Kingdom. Journal of Clinical Microbiology. 57(6): e00020-19.

Clinical and Laboratory Standards Institute (2017). Performance Standards for Antimicrobial Susceptibility Testing. CLSI Supplement M100. 27th ed. Wayne, PA.

Cuny C, Layer F, Strommenger B, & Witte W (2011). Rare occurrence of methicillin-resistant Staphylococcus aureus CC130 with a novel mecA homologue in humans in Germany. PloS One. 6(9): e24360.

David M Z & Daum R S (2010). Community-associated methicillin-resistant Staphylococcus aureus: epidemiology and clinical consequences of an emerging epidemic. Clinical Microbiology Reviews. 23(3): 616–687.

Donkor ES, Dayie NT, & Tette EM (2019). Methicillin-resistant Staphylococcus aureus in Ghana: Past, present, and future. Microbial Drug Resistance. 25(5): 717-724.

EF K, JK J, & MA J-R (2016). Community-associated methicillin-resistant Staphylococcus aureus: an enemy amidst us. PLoS Pathogens. 12(10): e1005837.

Elkholy MT & Ewees IE (2010). Mobile (cellular) phones contamination with nosocomial pathogens in intensive care unit. Medical Journal of Cairo University. 78(2): 1-5.

Ezeamagu C, Imanatue I, Dosunmu M, Odeseye A, Baysah G, Aina D, Odutayo F, & Mensah-Agyei G (2018). Detection of methicillin resistant and toxin-associated genes in Staphylococcus aureus. Beni-Suef University Journal of Basic and Applied Sciences. 7(1): 92-97.

Furuhata K, Ishizaki N, Sogawa K, Kawakami Y, Lee S I, Sato M, & Fukuyama M (2016). Isolation, identification and antibacterial susceptibility of Staphylococcus spp. Associated with the mobile phones of university students. Biocontrol Science. 21(2): 91-98.

Garcia-Alvarez L, Holden MTG, Lindsay H, Webb CR, Brown DFJ, Curran MD, Walpol E, Brooks K, Pickard DJ, Teale C, Parkihll J, Bentley SD, Edwards GF, Girvan EK, Kearns AM, Pichon B, Hill RLR, Larsen AR, Skov RL, Peacock SJ, Maskell DJ, & Holmes MA (2011). Methicillin-resistant Staphylococcus aureus with a novel mecA homologue in human and bovine populations in the UK and Denmark: a descriptive study. Lancet Infectious Diseases. 11: 595–603.

Hassoun A, Vellozzi EM, & Smith MA (2004). Colonization of personal digital assistants carried by healthcare professionals. Infection Control and Hospital Epidemiology. 25(11): 1000-1001.

Heyba M, Ismaiel M, Alotaibi A, Mahmoud M, Baqer H, Safar A, Al-Sweih N, & Al-Taiar A (2015). Microbiological contamination of mobile phones of clinicians in intensive care units and neonatal care units in public hospitals in Kuwait. BMC Infectious Diseases. 15:434. Doi: 10.1186/s12879-015-1172-9.

Kateete DP, Bwanga F, Seni J, Mayanja R, Kigozi E, Mujuni B, Ashaba FK, Baluku H, Najjuka CF, Kallander K, Rutebemberwa E, Asiimwe BB & Joloba ML (2019). CA-MRSA and HA-MRSA coexist in community and hospital settings in Uganda. Antimicrobial Resistance and Infection Control. 8: 94. Doi: 10.1186/s13756-019-0551-1.

Kolawole DO, Adeyanju A, Schaumburg F, Akinyoola AL, Lawal OO, Amusa YB, Kock R, & Becker K (2013). Characterization of colonizing Staphylococcus aureus Isolated from surgical wards’ patients in a Nigerian University Hospital. PLoS One. 8(7): e68721. doi.org/10.1371/journal.pone.0068721.

Kong C, Neoh H, & Nathan S (2016). Targeting Staphylococcus aureus toxins: a potential form of anti-virulence therapy. Toxins. 8: 72.

Lakhundi S & Zhang K (2018). Methicillin-resistant Staphylococcus aureus: molecular characterization, evolution, and epidemiology. Clinical Microbiology Reviews. 31(4): e00020-18.

Liu M, Liu J, Guo Y, & Zhang Z (2010). Characterization of virulence factors and genetic background of Staphylococcus aureus isolated from Peking University People’s Hospital between 2005 and 2009. Current Microbiology. 6: 435–443.

Monecke S, Coombs G, Shore AC, Coleman DC, Akpaka P, Borg M, Chow H, Lp M, Jatzwauk L, Jonas D, Kadiec K, Keams A, Laurent F, O’Brien FG, Pearson J, Ruppelt A, Schwarz S, Scicluna E, Slickers P, Tan H, Weber S, & Ehricht R (2011). A field guide to pandemic, epidemic and sporadic clones of methicillin-resistant Staphylococcus aureus. PloS One. 6(4): e17936. Doi: 10.1371/journal.pone.0017936.

Naimi HM, Rasekh H, Noori AZ, & Bahaduri MA (2017). Determination of antimicrobial susceptibility patterns in Staphylococcus aureus strains recovered from patients at two main health facilities in Kabul, Afghanistan. BMC Infectious Diseases. 17(1): 1-7.

Neubauer G, Rooslim KN, & Wiart J (2005). Study on feasibility of epidemiological studies on health effects mobile telephone stations. Journal of Health and Population. 20(4): 200-210.

Nwankwo EO, Ekwunife N, & Mofolorunsho KC (2014). Nosocomial pathogens associated with the mobile phones of healthcare workers in a hospital in Anyigba, Kogi state, Nigeria. Journal of Epidemiology and Global Health. 4(2): 135-140.

Odiete J, Olayiwola JO, & Abiala MA (2018). Mobile phones as fomites for the transmission of antibiotic resistant bacteria in the rural community. Academia Journal of Microbiology Research. 6: 023-027. DOI: 10.15413/ajmr.2018.0102

Paterson GK, Harrison EM & Holmes MA (2014). The emergence of mecC methicillin-resistant Staphylococcus aureus. Trends in Microbiology. 22(1): 42-47.

Prosperi M, Veras N, Azarian T, Rathore M, Nolan D, Rand K, Cool RL, Johnson J, Morris Jnr JG & Salemi M (2013). Molecular epidemiology of community associated methicillin-resistant Staphylococcus aureus in the genomic era: across-sectional study. Scientific Reports. 3:1902. Doi.org/10.1038/srep01902.

Reynolds KA, Watt PM, Boone SA, & Gerba CP (2005). Occurrence of bacteria and biochemical markers on public surfaces. International Journal of Environmental Health Research. 15(3): 225-234.

Tong SY, Davis JS, Eichenberger E, Holland TL, & Fowler VG (2015). Staphylococcus aureus infections: epidemiology, pathophysiology, clinical manifestations, and management. Clinical Microbiology Reviews. 28: 603–661.

Turner NA, Sharma-Kuinkel BK, Maskarinec SA, Eichenberger EM, Shah PP, Carugati M, Holland TL, & Fowler Jr VG (2019). Methicillin-resistant Staphylococcus aureus: an overview of basic and clinical research. Nature Reviews Microbiology. 17(4): 203-218.

Ustun C & Cihangiroglu M (2012). Health care workers’ mobile phones: a potential cause of microbial cross-contamination between hospitals and community. Journal of Occupational and Environmental Hygiene. 9(9): 538-542.

Ya’aba Y, Chuku A, Okposhi US, Hadi NS & Ramalan SA (2020). Bacterial contamination associated with mobile cell phones among undergraduate students of Federal University of Lafia, Nasarawa State, Nigeria. Nigerian Journal of Basic and Applied Sciences. 28(2): 36-42.

Yusha M, Bello M, & Sule H (2010). Isolation of bacteria and fungi from personal and public mobile cell phones: A case study of Bayero University, Kano (old campus). International Journal of Biomedical and Health Science. 6: 97-102.

Zakai S, Mashat A, Abumohssin A, Samarkandi A, Almaghrabi B, Barradah H, & Jiman-Fatani A (2016). Bacterial contamination of cell phones of medical students at King Abdulaziz University, Jeddah, Saudi Arabia. Journal of Microscopy and Ultrastructure. 4(3): 143-146.

Zhan XY & Zhu QY (2018). Evolution of methicillin-resistant Staphylococcus aureus: evidence of positive selection in a penicillin-binding protein (PBP) 2a coding gene mecA, Infection, Genetic and Evolution. 59: 16-22. Doi: 10.1016/j.meegid.2018.01.018.


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