Chemical composition and Larvicidal efficacy of Ficus sycomorus leaf extract against major malaria vector Anopheles coluzzii
), Chioma A. Anosike(2), Lawrence U. S. Ezeanyika(3), Parker J. Elijah(4), Hafeez M. Yakasai(5), Abdurrazak Muhammad(6), Abdullahi Muhammad(7), Sulaiman S. Ibrahim(8),
(1) Bayero University, Kano
(2) University of Nigeria, Nsukka
(3) University of Nigeria, Nsukka
(4) University of Nigeria, Nsukka
(5) Bayero University
(6) Skyline University
(7) Bayero University, Kano
(8) Bayero University
Corresponding Author
Abstract
Resistance to synthetic insecticides used in control of malaria vectors is a major threat to malaria control globally. Natural insecticides of plant origin provide environmentally safe alternativess. This study characterized the phytochemical contents of the Ficus sycomorus leaves and insecticidal activities of its methanolic extract on Anopheles colluzzii larvae. The active secondary metabolites from methanol extract of F. sycomorus leaves were characterized using gas chromatography - mass spectrometry (GC-MS). A modified WHO standard protocol for larvicidal bioassay was used to determine the activity of methanol extract of the F. sycomorus. Molecular species identification showed that 75 % of the larvae were Anopheles coluzzii (Coetzee & Wilkerson); 6.25 % Anopheles arabiensis and 12.5 % Anopheles gambiae s.s. The extract reduced the survival of An. coluzzii larvae by approximately 52 % at LC50 of 0.225 mg/ml (95% CI: 0.197-0.234; R2 =0.9445) after 24 h, and the mortality was dose dependent (with highest mortality of 51.67 % at 1 mg/ml). Phytochemical screening revealed the presence of alkaloids, flavonoids, tannins, saponins, terpenoids, phytosterols and phenols, with alkaloid and flavonoids having the highest concentration in the extract. The GC-MS revealed that the extract contained: tetrapentacontane, 66.6 %; stigmasterol, 2.81 %; squalene, 1.01 %; bis(2-ethylhexyl) phthalate, 4.04 %; and 4,8,12,16-tetramethylheptadecan-4-olide, 0.51 %. The toxicity of the extract may be due to different insecticidal phytochemicals, probably working independently, synergistically, or additively. Overall, the F. sycomorus leaf extract is a promising larvicide against malarial vector An. coluzzii and should be exploited as an alternative, environmentally safe larvicide.
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References
Abbott WS (1925) A method of computing the effectiveness of an insecticide. J. of Economic Entomol., 18: 265-267.
Afaf MW, Afaf AMB, & Hossain MA (2015). Evaluation of antioxidant and antimicrobial potential of different leaves crude extracts of Omani Ficus carica against food borne pathogenic Bacteria. Asian Pac. J. Trop. Dis. 5 (1): 13–16.
Ahmad M, Denholm I, & Bromilow RH (2006). Delayed cuticular penetration and enhanced metabolism of deltamethrin in pyrethroid-resistant strains of Helicoverpa armigera from China and Pakistan. Pest Manag Sci., 62:805-810.
Anosike CA, Babandi A, & Ezeanyika LUS (2021). Potentiation Effects of Ficus sycomorus Active Fraction Against Permethrin-Resistant Field-Population of Anopheles coluzzii (Diptera: Culicidae) Neotrop. Entomol.; 50(3): 484-496 (2021) https://doi.org/10.1007/s13744-021-00858-2
Ayyanar M & Ignacimuthu S (2009). Herbal medicines for wound healing among tribal people in Southern India: Ethnobotanical and scientific evidences. Int. J. App. Res Nat. Prod. 2(3):24-49
Babandi A, Muhammad MA, Anosike CA, & Ezeanyika LUS (2019) In vitro Anti-malarial Potential of Chloroform Fruit Crude Extract of Ficus sycomorus L. against Blood-stage Plasmodium falciparum. Nig Res J. Chem Sci. 6:33-48.
Babandi A, Anosike CA, & Lawrence USE (2021). Larvicidal and synergistic toxicity of Ficus sycomorus and Calotropis procera leaf extracts against malarial vector Anopheles gambiae complex from Kano-Nigeria: A green bio-control approach. Asian J. Green Chem, 5(3):248-262. DOI: 10.22034/ajgc.2021.262853.1290
Bagavan A, Rahuman AA, Kamaraj C, & Geetha K (2008). Larvicidal activity of saponin from Achyranthes aspera against Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae). Parasit Res, 103(1): 223-229. doi:10.1007/s00436-008- 0962-z.
Barbehenn RV & Constabel PC (2011). Tannins in plant–herbivore interactions. Phytochemistry, 72(13):1551–1565.
Becker N, Petric D, Boase C, Lane J, Zgomba M, Dahl C, & Kaiser A. (2010). Mosquitoes and their control. Heidelberg: Springer Verlag; 2: 2010.
Benelli G, Maggi F, & Pavela R (2018) Mosquito control with green nanopesticides: towards the One Health approach? A review of non-target effects. Environ Sci Pollut Res. 25: 10184–10206.
Bhalerao SA & Sharma AS (2014). Ethnomedicinal, phytochemical and pharmacological profile of Ficus religiosa Roxb. Int. J. Curr Microbiol App. Sci. 3(11): 528-538.
Cavalcanti ESB, de Morais SM, Ashley ALM, & William PSE (2004). Larvicidal activity of essential oils from Brazilian plants against Aedes aegypti L. Memo´ rias do Instituto Oswaldo Cruz. 99:541–544.
Cavalcante GM, Moreira AFC, & Vasconcelos SD (2006) Potencialidade inseticida de extratos aquosos de essências florestais sobre mosca-branca. Pesquisa Agropecuária Brasileira, 41(1):9-14. doi: 10.1590/S0100- 204X2006000100002.
Chogo J & Crank G (1981). Chemical composition and biological activity of the Tanzanian plant Ocimum suave. J. Nat Prod.;44(3):308–11.
Dias CN & Moraes DFC (2014). Essential oils and their compounds as Aedes aegypti L. (Diptera: Cilicidae) larvicides: Review. Parasitol. Res., 113:565–592.
Ferrer I & Thurman EM (2003). Liquid Chromatography Mass Spectrometry/Mass Spectrometry, MS/MS and Time-of-Flight MS: Analysis of Emerging Contaminants, American Chemical Society Symposium Series 850, Oxford University Press, New York, USA, 2003.
Finney DJ (1971). Probit analysis, 3rd ed. Cambridge University Press, Cambridge. Pp 68–72.
Frazier AR, Deaville ER, Green RJ, Stringano E, Willoughby I, Plant J, & Mueler-Harvey I (2010). Interactions of tea tannins and condensed tannins with proteins. J. pharm Biomed analysis 51:490-495.
Gillies MT & Coetzee M (1987). A supplement to anophelinae of Africa south of Sahara (Afro-tropical region). The South African Inst. for Med Res. 55:96–110.
Govindarajan M (2010). Larvicidal and repellent properties of some essential oils against Culex tritaeniorhynchus Giles and Anopheles subpictus Grassi (Diptera: Culicidae). Asian Pac J. Trop Med.; 4:106–111.
Grisales N, Poupardin R, Gomez S, Fonseca-Gonzalez I, Ranson H, & Lenhart A (2013). Temephos resistance in Aedes aegypti in Colombia compromises dengue vector control. PLoS Neg. Trop. Dis. 7. http://dx.doi.org/10.1371/journal.pntd.
Gueye S, Diop MT, Seck D, & Sembene M (2011). Biochemical fractions activity of Annona senegalensis Pers. Extract leaves to protect groundnut against the seed-beetle Caryedon serratus Ol. (Coleoptera, Chrysomelidea, Bruchinae). Int J Plant Animal Environ Sci; 1(2):122-130.
Hikal WM, Baeshen RS, Said-Al Ahl HAH (2017). Botanical insecticide as simple extractives for pest control. Cogent Biol., 3: 1404274.
Hostettmann K & Marston A (1995). Saponins (Chemistry and pharmacology of natural products). University Press, Cambridge, 1995; 132.
Houghton PJ, Ren Y, Howes MJ (2006). Acetylcholinesterase inhibitors from plants and fungi. Nat Prod Rep; 23: 181-99.
Ibrahim SS, Yayo AM, Tukur Z, Irving H, & Wondji CS (2014). High frequency of kdr L1014F is associated with pyrethroid resistance in Anopheles coluzzii in Sudan savannah of northern Nigeria. BMC Infectious Dis 14: 441.
Ibrahim SS, Riveron JM, Robert S, Helen I, & Charles SW (2016). The Cytochrome P450 CYP6P4 Is Responsible for The High Pyrethroid Resistance In Knockdown Resistance-Free Anopheles Arabiensis. Insect Biochem Mol Biol., 68: 23e32. http://dx.doi.org/10.1016/j.ibmb.2015.10.015.
Ihsan U, Abdul LK, Liaqat A, Abdur, Rahim K, Muhammad W, In-Jung L, & Jae-Ho S (2014) An insecticidal compound produced by an insect pathogenic bacterium suppresses host defenses through phenoloxidase Inhibition. Molecules. 19(12):20913- 20928.
Jacob MR, Helen I, Miranda N, Kayla GB, Sulaiman SI, Mark JIP, & Charles SW (2013). Directionally selected cytochrome P450 alleles are driving the spread of pyrethroid resistance in the major malaria vector Anopheles funestus. PNAS, 110 (1): 252-257.
Jang YS, Kim MK, Ahn YJ, & Lee HS (2002). Larvicidal activity of Brazilian plants against Aedes aegypti and Culex pipiens pallens (Diptera: Culicidae). Agric Chem. Biotechnol. 44: 23–26.
Kilickaya SE, Usta A, & Akiner MM (2019). Larvicidal activity of some medicinal plant naturally growing in turkey against Aedes albopictus (Diptera: culicidae. J. Anatolian Environml Anim. Sci. 4(1), 53-59.
Kotkar, HM, Mendki PS, Sadan SVG, Jha SR, Upasani SM, & Maheshwari VL (2002) Antimicrobial and pesticidal activity of partially purified flavonoids of Annona squamosa. Pest Manag. Sci., 58: 33-37.
Kotzekidou R, Giannakidis P, & Boulamatsis A (2008). Antimicrobial activity of some plant extracts and essential oils against food borne pathogens in vitro and on the fate of inoculated pathogen in chocolate. Food Sci. Tech., 41:119-127.
Lagunes TA (1994). Plant extracts and Powders and Minerals against pests of corn and beans for subsistence Agriculture. Montecillo: College-Graduate/USAID CONACYT/ BORUCONSA, P 35.
Lin Y, Jin T, Zeng L, & Lu Y (2012). Cuticular penetration of b-cypermethrin in insecticide-susceptible and resistant strains of Bactrocera dorsalis. Pestic Biochem Physiol., 103:189-193.
Livak KJ (1984). Organization and Mapping of a sequence On the Drosophila-Melanogaster X-Chromosome and Y-Chromosome That Is Transcribed during spermatogenesis. Genetics, 107: 611-634.
Mahmoud EH, Bashir HN, & Assad OY (2017). Effect of Basil (Ocimum basillicum) leaves powder and ethanolic extract on the 3rd larval instar of Anopheles arabiensis (patton 1905) (Culicidae: Diptera). Int. J. Mosquito Res., 4(2): 52-56.
Mithofer A & Boland W (2012). Plant defense against herbivores: chemical aspects. Ann Rev of Plant Biol., 63: 431-450.
Mollar-Jacobs LL, Murdock CC, & Thomas MB (2014). Capacity of mosquitoes to transmit malaria depend on larval environment. Parasites and Vectors, 7:593. Doi: 1186/s13071-0140593-4.
Muhammad A, Ibrahim SS, Mukhtar MM, Irving H, Abajue MC, Edith NMA et al. (2021). High pyrethroid/DDT resistance in major malaria vector Anopheles coluzzii from Niger-Delta of Nigeria is probably driven by metabolic resistance mechanisms. PLoS ONE 16(3):e0247944. https:// doi.org/10.1371/journal.pone.0247944
Muhammad SN (2012). Effects of plant extracts on the behaviour and physiology of the Odontotermes obesus (Ramb.) (Isoptera: Termitidae). PhD thesis, University of Agriculture, Faisalabad, Pakistan. 129pp.
Mukandiwa L, Eloff JN, & Naidoo V (2015) Larvicidal activity of leaf extracts and seselin from Clausena anisata (Rutaceae) against Aedes aegypti. South Afr J. of Bot. 100: 169–173.
Naqqash MN, Gokce A, Bakhsh A, & Salim M (2016). Insecticide resistance and its molecular basis in urban insect pests. Parasitol Res.; 115:1363–1373.
Nkya TE, Akhouayri I, Kisinza W, & David J (2012). Impact of environment on mosquito response to pyrethroid insecticides: Facts, evidences and prospects. Insect Biochem Mol Biol.; 43: 410.
Paiva PMG, Santana GMS, Souza IFAC, Albuquerque LP, Agra-Neto AC, Albuquerque AC, Luz LA, Napoleao, TH, & Coelho LCBB (2010). Effect of lectins from Opuntia ficus indica cladodes and Moringa oleifera seeds on survival of Nasutitermes corniger. Int Biodet Biodeg, 30: 1 - 8.
Procopio TF, Fernandes KM, Pontual EV, Ximenes RM, de Oliveira ARC, Souza CDS, & Napoleao TH (2015). Schinus terebinthifolius leaf extract causes midgut damage, interfering with survival and development of Aedes aegypti larvae. PLoS One, 10(5): e0126612.
Rajasingh R, Ahmed F, Pandeeswari M, Reegan D, & Tennyson S (2017). Laboratory evaluation of few plant extracts for their ovicidal, larvicidal and pupacidal activity against medically important human dengue, chikungunya and zika virus vector, Aedes aegypti Linnaeus 1762 (Diptera: Culicidae). Indian J Mosquito Res. 4(4): 17- 28.
Rajeswary M, Govindarajan M, Murugan K, Hwang JS, Barnard DR, Amsath A et al. (2014). Ovicidal efficacy of Ageratina adenophora (Family: Asteraceae) against Anopheles stephensi (Diptera: Culicidae). Int. J. Pure and Appld Zoo., 2(3): 196–199.
Ranson H, Burhani J, Lumjuan N, & Black WC (2010). Insecticide resistance in dengue vectors. Trends in Parasitol, 27: 91–98.
Ranson H & Lissenden N (2016). Insecticide resistance in African Anopheles mosquitoes: a worsening situation that needs urgent action to maintain malaria control. Trends Parasitol., 32:187–96.
Rautela I, Dheer P, Thapliyal P, Joshi T, Sharma N, & Sharma MD (2018). GC-MS analysis of plant leaf extract of Datura stramonium in different solvent system. Eur J Biomed Pharm Sci, 5(10): 236-245.
Rhome AA (2013) Phytochemicals from Ficus sycomorus L. leaves act as insecticides and acaricides. African J Agric Res 8(27):3571–9. doi: 10.5897/AJAR2013.7243.
Santolamazza F, Calzetta M, Etang J, Barrese E, Dia I, Caccone A, Donnelly MJ, Petrarca V, Simard F, Pinto J, & Torre AD (2008). Distribution of knock-down resistance mutations in Anopheles gambiae molecular forms in west and west-central Africa. Malar J, 7: 74-10.1186/1475-2875-7-74.
Santos SRL, Melo MA, Cardoso AV, Santos RLC, de Sousa DP, Cavalcanti SCH (2011). Structure-activity relationships of larvicidal monoterpenes and derivatives against Aedes aegypti Linn, Chemosphere 84: 150–153.
Scotti L, Scotti MT, Silva VB, Santos SRL, Cavalcanti SCH, Mendonça Jr FJB (2014). Chemometric studies on potential larvicidal compounds against Aedes aegypti. Med Chem., 10:201–210.
Sheikha KM, Ruqaiya NSW, & Hossain MA (2015). In vitro evaluation of the total phenolic and flavonoid contents and the antimicrobial and cytotoxicity activities of crude fruit extracts with different polarities from Ficus sycomorus. Pac. Sci. Rev. A Nat. Sci. Eng., 17:103–108.
Svoboda KP & Hampson J (1999). Bioactivity of essential oil of selected temperate aromatic plants: antibacterial, antioxidant, anti-inflammatory and other related pharmacological activities. In Speciality chemicals for the 21st century, ADEME/IENICA Seminar, 16–17 Sep 1999. ADEME, Paris, pp. 43-49.
Tehri K & Singh N (2015). The role of botanicals as green pesticides in integrated mosquito management – a review. Int J Mosq Res., 2:18–23.
Timmel MA, Byl JAW, & Osheroff N (2013). Epimerization of green tea catechins during brewing does not affect the ability to poison human type II topoisomerases. Chem Res Toxicol, 26: 622–8.
Tiwari P, Kumar B, Kaur M, Gurpreet K, & Harleen K (2011). Phytochemical screening and extraction: A review, Int. Pharm. Sci, 1: 98-106.
Wang XL, Lin QX, Wang J, Lu XG, & Wang GP (2013). Effect of wetland reclamation and tillage conversion on accumulation and distribution of phthalate esters residues in soils. Ecol Eng 51:10–15. doi:10.1016/j.ecoleng.2012.12.079.
Wang H, Lai D, Yuan M, & Xu, H (2014). Growth inhibition and differences in protein profiles in azadirachtin-treated Drosophila melanogaster larvae. Electrophoresis 35(8):1122–1129.
Wood O, Hanrahan S, Coetzee M, Koekemoer L, & Brooke B (2010). "Cuticle thickening associated with pyrethroid resistance in the major malaria vector Anopheles funestus." Parasites & Vectors 3(1): 67.
World Health Organization, WHO (2018). World malaria report (2018) World Health Organization, Geneva. http://www.who.int/malaria/world_malaria_report_2018/en/
World Health Organization, WHO (2005). Test procedures for insecticide resistance monitoring in malaria vectors. Bio-efficacy and persistence of insecticides on treated surfaces.
WHO/MAL/9812 Report of the WHO Informal Consultation. Geneva: World Health Organization.
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