1. Home
  2. Vol 13, No 2s (2020)
  3. Ajani

ORIGINAL RESEARCH ARTICLE - Biomarker profile of a silver catfish Chrysicthys Nigrodigitatus in heavy metal polluted areas of the Lagos Lagoon, Nigeria

Gloria E. Ajani(1),


(1) Nigerian Institute for Oceanography and Marine Research
Corresponding Author

Abstract


Aquatic ecosystems are usually exposed to contaminants including heavy metals from anthropogenic activities and high concentration of these metals may alter physiological function in fish muscle. Assessment of biomarker profile will provide the role of antioxidant defence enzymes in response to metal accumulation. The present study aims to find the relationship between biomarker pattern in fish muscle and the contribution of antioxidant defence enzymes to sustain balance from metal toxicity. Adult fish (Chrysicthys nigrodigitatus), water and sediment samples were taken from five sampling stations, during the wet and dry season in the Lagos Lagoon. Heavy metals (lead, cadmium, chromium, copper, zinc, iron) levels, antioxidant defence enzyme activity (superoxide dismutase, catalase, glutathione peroxidase, reduced glutathione) and malondialdehyde were analysed in fish muscle using standards methods. Heavy metal levels showed significant difference (p<0.05) in fish muscles, water and sediment, with values above recommended standard for aquatic life. PCA showed that elevated activities of antioxidant defence enzymes correlated with increase metal concentrations within the fish muscle, which may indicate possible oxidative stress. Hence, measures should be implemented and sustained in the treatment of effluents before discharging into the aquatic habitat so as to reduce stress and sustained the wild population.

Keywords


Chrysicthys nigrodigitatus, heavy metals, antioxidant defence enzymes

References


Lopez-Galindo C, Vargas-Chacoff L, Nebot E, Casanueva J F, Rubio D, Sole M, & Mancera J M (2010). Biomarker responses in Solea senegalensis exposed to sodium hypochlorite used as antifouling. Chemosphere. 78: 885.

Kroon F, Streten C, & Harries S (2017). A protocol for identifying suitable biomarkers to assess fish health: A systematic review. PLoS ONE 12(4): e0174762.

Fakhri M (2012). The state of the Lebanese coastal waters, in: Review and Perspectives of Environmental Studies in Lebanon. INCAM-EU/ CNRS Lebanon, pp. 31–54

Rather M, MohdTilwani Y, & Dey A (2019). Assessment of heavy metal contamination in two edible fish species Carassius and Triplophysa kashmirensis of dal Lake, Srinagar, Kashmir, India. Environ Monit Assess 2:191–242.

Tchounwou P, Yedjou C, Patlolla A K, & Sutton D J (2012). Heavy Metals Toxicity and the Environment. National Institutes of Health. Public Access. 101: 133-164.

Duffus J H (2002). Heavy metals-a meaningless term? Pure and Applied Chemistry. 74(5):793–807.

Moniruzzaman M, Midday P, Dhara A, Das D, Ghosal L, Mukherjee D, & Chakraborty S B (2017). Change in redox state and heat shock protein expression in an Indian major carpcirrhosis exposed to zinc and lead. Journal of Toxicological Science. 42. 6., 731-740.

Akporido S O & Onianwa P C (2015). Heavy metals and total petroleum hydrocarbon concentrations in surface water of Esi River, Western Niger Delta. Research Journal of Environmental Sciences. 9: 88-100.

Das D, Moniruzzaman M, Sarbajna A, & Chakraborty S (2017). Effect of heavy metals on tissue-specific antioxidant response in Indian major carps. Environmental Science and Pollution Research. 24: 18010–18024.

Adeogun A O & Chukwuka A V (2012). Toxicity of industrial wastewater acting singly or in joint ratios on Clarias gariepinus. American Journal of Environmental Science. 8: 366-375.

Savorelli F, Manfra L, Croppo M, Tornambe A, Palazzi D, Canepa S, Trentini P, Cicero A, & Faggio C (2017). Fitness Evaluation of Ruditapesmphilippinarum Exposed to Ni. Biological Trace Element Research. 177: 384–393.

Akintujoye J F, Anumudu C I, & Awobode H O. Assessment of heavy metal residues in water, fish tissue and human blood from Ubeji, Warri, Delta State, Nigeria. Journal of Applied Science and Environmental Management. 2; 17(2):291-297.

Ibanga B, Nkwoji B, Usese I, Onyema C, & Chukwu O (2019). Hydrochemistry and heavy metals concentrations in sediment of Woji creek and Bonny estuary, Niger Delta, Nigeria, Regional Studies in Marine Science. 25: 1004-10436.

Mukherkee J, Moniruzzaman M, Bhusan S, Lek S, & Ray S (2017). Towards a physiological response of fishes under variable environmental conditions: An approach through neural network. Ecological indicators. 78: 381-394.

van der Oost R, Beyer J, & Vermeulen N P E (2003). Fish bioaccumulation and biomarkers in environmental risk assessment: a review. Environ ToxicolPharmacol. 13: 57–149.

Kroon F, Streten C, & Harries S (2017). A protocol for identifying suitable biomarkers to assess fish health: A systematic review. PLoS ONE 12(4): e0174762

Tchounwou P, Yedjou, C G, Patlolla A K, & Sutton D. J. (2012). Heavy Metals Toxicity and the Environment. National Institutes of Health. Public Access. 101: 133-164.

Akporido S O & Onianwa P C (2015). Heavy metals and total petroleum hydrocarbon concentrations in surface water of Esi River, Western Niger Delta. Research Journal of Environmental Sciences. 9: 88-100.

Adeogun A O & Chukwuka A V (2012). Toxicity of industrial wastewater acting singly or in joint ratios on Clarias gariepinus. American Journal of Environmental Science. 8: 366-375.

Savorelli F, Manfra L, Croppo M, Tornambe A, Palazzi D, Canepa S, Trentini P, Cicero A, & Faggio C (2017). Fitness Evaluation of Ruditapes philippinarum Exposed to Ni. Biological Trace Element Research. 177: 384–393.

Akintujoye J F, Anumudu C I, Awobode H O. Assessment of heavy metal residues in water, fish tissue and human blood from Ubeji, Warri, Delta State, Nigeria. Journal of Applied Science and Environmental Management. 25; 17(2):291-297.

Ibanga B, Nkwoji B, Usese I, Onyema, C, & Chukwu O (2019). Hydrochemistry and heavy metals concentrations in sediment of Woji creek and Bonny estuary, Niger Delta, Nigeria, Regional Studies in Marine Science. 25: 1004-10436.

Mukherkee J, Moniruzzaman M, Bhusan S, Lek S, & Ray S (2017). Towards a physiological response of fishes under variable environmental conditions: An approach through neural network. Ecological indicators. 78: 381-394.

Schlenk D. 1999. Necessity of Defining Biomarkers for Use in Ecological Risk Assessments. Marine Pollution Bulletin. 39: 48–53.

Handy R D, Galloway T S, & Depledge M H. (2003). A proposal for the use of biomarkers for the assessment of chronic pollution and in regulatory toxicology. Ecotoxicology. 12: 331–43.

Storelli, M (2008). Potential human health risks from metals (Hg, Cd, and Pb) and polychlorinated biphenyls (PCBs) via seafood consumption: Estimation of target hazard quotients (THQs) and toxic equivalents (TEQs). Food and chemical toxicity. 46: 2782-2788.

Moniruzzaman M, Midday P, Dhara A, Das D, Ghosal I, Mukherjee D, & Chakraborty S B (2017). Change in redox state and heat shock protein expression in an Indian major carp Cirrhinuscirrhosus exposed to zinc and lead. Journal of Toxicological Science. 42: 731-740.

Ajani E G (2014). Oxidative stress in fish living in coastal water polluted with sawdust and wood waste along Lagos Lagoon, Nigeria. Researcher 6: 1–5.

Akinsanya B, Otubanjo O A & Ibidapo C A (2007). Helminth bioload of Chrysichthys nigrodigitatus (Lacepede 1802) from Lekki Lagoon, Lagos Nigeria. Turkish Journal of Fisheries and Aquatic Sciences. 7:83-87.

Omondi R, Yasindi A W, & Magana A M (2011). Spatial and temporal variations of zooplankton in relation to some environmental factors in lake Baringo, Kenya. Egerton Journal of Science and Technology. 11:29-50.

Esenowo I K, Ugwumba A, Umoh I, & Andem A B (2017). Aspects of the Biology of Silver Catfish (Chrysichthys nigrodigitatus) in Nwaniba River, Southeast Nigeria. Asian Journal of Biology. 3: 1-9.

Webb J E (1958). The ecology of Lagos Lagoon, some physical properties of lagoon deposits. Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences. 683: 393-419.

Ajao E & Fagade S O (1990). A study of sediments in communities in Lagos Lagoon. Oil and Chemical. 785-117.

IPEP (2006). The international POPs Elimination Projects (IPEP). Assessment of the Lagos Lagoon for POPs sources, types and impacts. Friend of the Environment (FOTE), Nigeria-Anglophone Africa, pp 123.

Oyedepo S O & Fagbenle R L 2011. A study on implementation of preventive maintenance programme in Nigeria power industry- Egbin thermal power plant, case study, Energy and Power Engineering. 3:207-220.

Idodo-Umeh G (2002). Pollution assessments of Olomoro water bodies using Physical, Chemical and Biological indices: PhD. Thesis, University of Benin, Benin City, Nigeria. pp 485.

Food and Agriculture Organization of the United Nation (FAO)/SIDA (2003). Manual of methods in aquatic environment research part 9. Analysis of metals and organochlorines in fish. FAO Fish Tech. Pap. (212) pp 32.

Kingston H M & Jassie L B (1988). Fundamental relationships in acid decomposition of samples for elemental analysis using microwave energy. Materials Research Society. 4:25.

Magwere T, Naiks Y S, & Hasler J A (1997). Effect of chloroquine treatment on antioxidant enzymes in rat liver and kidney. Free Radical Biology and Medicine. 22: 321-327.

McCord J & Fridovich I (1969). Superoxide dismutase, an enzymatic function for erythrocuprein. Journal of Biological Chemistry. 244: 6049–6055.

Aebi H. (1984) Catalase in vitro. In Methods in enzymology Jan 1 (Vol. 105, 316 pp. 121-126). Academic Press.

Lamprecht W & Trautschold I (1963). in Methods of Enzymatic Analysis, eds Bergmeyer H U (Academic Press, New York & London), pp 453.

Ellman G L & Fiches F T (1959). Quantitative determination of peptides by sulfhydryl groups. Archives of Biochemistry and Biophysics. 82: 70-72.

Weckbercker G & Cory J G (1988). Ribonucleotide reductase activity and growth of glutathione-depended mouse leukaemia L 1210 cells in vitro. Cancer Letters. 40: 257-264.

Buege J A & Aust S D (1978). Microsomal lipid peroxidation Methods. Enzymol52: 302-310.

Smith P K, Krohn R I, Hermanson G T, Mallia A K, Gartner F H, Provenzano M D, Fujimoto E K, Goeke N M, Olson B J, & Klenk D C (1985). Measurement of protein using bicinchoninic acid. Analytical Biochemistry. 150.1: 76-85.

Food and Agriculture Organization of the United Nation (FAO)/WHO (2016): Joint FAO/WHO food standards programme codex committee on contaminants in foods, fifth. Session pp 64-89

Aliko V, Qirjo M, Sula E, Morina V, & Faggio C. (2018). Antioxidant defense system, immune response and erythron profile modulation in goldfish, Carassius auratus, after acute manganese treatment. Fish and Shellfish Immunology. 76:101–109.

Deepali, K K (2010). Metals concentration in textile and tannery, associated soils and ground water. New York Science Journals. 3 (4), 82-8


Full Text: PDF

Article Metrics

Abstract View : 308 times
PDF Download : 94 times

DOI: 10.57046/XRQC1151

Refbacks

  • There are currently no refbacks.


Copyright (c) 2021 Proceedings of the Nigerian Academy of Science

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.