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Biosurfactant quality generated from fungi using agro-wastes (maize husk, rice husk, and augarcane bagasse)

Chukwuma S. Ezeonu(1), Amayindi Siman(2),


(1) Federal University Wukari, Taraba State, Nigeria
(2) Federal University Wukari, Taraba State, Nigeria
Corresponding Author

Abstract


Biosurfactants are amphiphilic compound that contain hydrophilic and hydrophobic moieties produced extracellularly by microorganism on cell surface or excreted extracellularly thereby reducing surface and interfacial tension between molecules at the surface and interface, respectively. This study was aimed on the isolation and identification of biosurfactant producing fungi; the production of biosurfactants from the identified fungi in a submerged fermentation using sugarcane bagasse, rice husk and maize husk as carbon sources and the evaluation of quality of biosurfactants generated. The biosurfactant production was assayed for 21 days with constant agitation for at most four times daily in an incubator (300C). The emulsification ability of the biosurfactant produced was analyzed on two hydrocarbons: diesel and kerosene. In diesel, the emulsification index (EI) showed highest in biosurfactant produced by Aspergillus niger in Rice Husk (BAR) after 72 hours (E72) (80.30±0.30%) and lowest in biosurfactant produced by Fusarum oxysporum in sugarcane bagasse after 24 hours (E24) (BFS) (33.43±0.10%). In kerosene, the highest emulsification index was observed in biosurfactant produced by Fusarum oxysporum in Maize Husk (BFM) E72 (94.25±0.25%) and lowest in biosurfactant produced by Fusarum oxysporum in sugarcane bagasse (BFS) E48 (52.53±0.10%). Therefore, comparing the biosurfactants produced by Fusarum oxysporum and Aspergillus niger, Fusarum oxysporum exhibited more emulsification ability than Aspergillus niger. The result of EI showed that Maize Husk and Rice Husk are good substrates for biosurfactant production while Fusarum oxysporum is the better biosurfactant producing fungi. In comparing the hydrocarbons, more emulsion was noticed in diesel than kerosene. The result of oil spreading assay with spent engine oil showed that biosurfactants produced by Fusarum oxysporum grown on Rice Husk had the highest area of displacement (13.88±0.01cm2) while biosurfactant produced by Aspergillus niger grown on Sugarcane Bagasse showed the least area of displacement (4.91±0.00cm2). The carbon sources used in this work are good for the production of biosurfactant. Upscaling of the process will yield biosurfactants applicable in industries.

Keywords


Agrowastes, Biosurfactants, Fungi.

References


Anyanwu CU, Obi SKC, & Okolo BN (2011). Lipopeptide biosurfactant production by Serratia marcescens NSK-1 strain isolated from petroleum contaminated soil. Journal of Applied Research. 7(1): 79-87.

Alexopoulos CJ (1962) Introductory Mycology. 2nd Edition. John Wiley and Sons, Inc., New York London. Sydney, pp: 119 – 159.

Chandran P & Das N (2010). Biosurfactant production and diesel oil degradation by yeast species Trichosporon asahii isolated from petroleum hydrocarbon contaminated soil. International Journal of Engineering Science and Technology. 2(12): 6942-6953.

Cheesbrough M (1984). District laboratory practice in tropical countries. Part 2. London: Cambridge University Press; p. 134–242.

Cooper DG & Goldenberg BG (1987) Surface active agents from two Bacillus sp. Applied Environmental Microbiology. 53:224-229.

Emine Y & Aysun E (2009). Screening the antimicrobial activity of biosurfactants produced by microorganisms isolated from refinery wastewaters. Journal of Applied Biological Sciences, 3(2): 163-168.

Green KR (1994). Studies on the epidemiology and control of yam anthracnose. Ph.D. Thesis, University of Reading, Reading, United Kingdom.

Govindammal M (2013). Investigation on antimicrobial activity of biosurfactant produced by Pseudomonas fluorescens isolated from mangrove ecosystem. International Research Journal of Pharmacy. 4(1): 230-232.

Kiran GS, Thomas TA, & Selvin J (2010). Production of a new glycolipid biosurfactant from marine Nocardiopsis lucentensis MSA04 in solid-state cultivation. Colloids and Surfaces. 78: 8–16.

Mahesh N, Murugesh S, & Mohana V (2006). Determination of the presence of biosurfactant produced by the Bacteria present in the soil samples. Research Journal of Microbiology. 1(4): 339-346.

Nelson PE, Tousson TA, & Marasssas WFO (1983) Fusarium species, an illustrated manual for identification. The Pennsylvania state press, University Park and London, pp: 193.

Okigbo RN, & Ikediugwu FEO (2000) Studies on biological control of postharvest rot of yam (Dioscorea sp.) with Trichoderma viride. Journal of Phytopathology.148:351–355.

Onyike RCI & Maduewesi JNC (1985). Variability in pathogenicity of isolates and sensitivity of dithane M–45 and PCNB on cocoyam rot Fungi. Nigerian Journal Plant Protect. 9:74–81.

Pornsunthorntawee O, Wongpanit P, Chavadej S, Abe M, & Rujiravanit R (2008) Structural and physicochemical characterization of crude biosurfactant produced by Pseudomonas aeruginosa SP4 isolated from petroleum-contaminated soil. Bioresource Technology. 99:1589–1595.

Rahman KSM, Rahman TJ, McClean S, Marchant R, & Banat IM (2002). Rhamnolipid biosurfactant production by strains of Pseudomonas aeruginosa using low-cost raw materials. Biotechnology Program, 18: 1277-1281.

Rippon JN (1988) The pathogenic fungi and pathogenic Actinomycetes. In: Medical mycology, 3rd edition. Publ. Sanders Co. Philadelphia, pp: 163.

Saharan BS, Sahu RK, & Sharma D (2011). A review of biosurfactant: fermentation, current developments, and perspectives. Genetic Engineering and Biotechnology, 29:1-14

Samson RA, Hockstra ES, & Van-Orschot AN (1984) Introductory to food borne fungi. The Netherlands academy of arts and science, pp: 1-25.

Snowdon AL (1991). A Colour atlas of postharvest diseases and disorders of fruits and vegetables UK. 2: 416.


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DOI: 10.57046/IRDN5640

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